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验证数据展示

  • Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    G65056-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    G65056-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for Single Cell
Cat No. G65056-1-5C

Print datasheet

CloneNo. UCHM-1

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell

Synonyms

CD14, CD14 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65056-1-5C targets CD14 in Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell Application Description
Tested Reactivity Human
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD14 (UCHM-1)
Synonyms CD14, CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_3673906
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCTTGCGTCTACCGCCCCCATATAAGAAA
Barcode SequenceCTTGCGTCTACCGCC
Form Liquid
UNIPROT IDP08571
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol