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MultiPro™ 5CFLX Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

CGTGGTTGGAAGCAC

CloneNo.

TS1/18

Cat no : G65190-1-5C

Print datasheet

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

验证数据展示

  • Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    G65190-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    G65190-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65190-1-5C on Resting PBMC

    G65190-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65190-1-5C on PBMC treated with 4hr LPS + TI

    G65190-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65190-1-5C targets CD18 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen Human Beta 2 Integrin 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD18 (TS1/18)
Synonyms CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC005861
Gene Symbol CD18
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCGTGGTTGGAAGCACCCCATATAAGAAA
Barcode SequenceCGTGGTTGGAAGCAC
Form Liquid
UNIPROT IDP05107
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol