验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
SINGLE CELL | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G65110-1-5C targets CD19 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
Tested Applications | Single Cell (Intra), Single Cell Application Description |
Tested Reactivity | Human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Oligo Conjugate |
Type | Monoclonal |
Full Name | MultiPro® 5CFLX Anti-Human CD19 (HIB19) |
Synonyms | B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12 |
Calculated Molecular Weight | 556 aa, 61 kDa |
GenBank Accession Number | BC006338 |
Gene Symbol | CD19 |
Gene ID (NCBI) | 930 |
ENSEMBL Gene ID | ENSG00000177455 |
RRID | AB_3673915 |
Conjugate | 5CFLX |
Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCGGCTGGTCCTTCATCCCATATAAGAAA |
Barcode Sequence | CGGCTGGTCCTTCAT |
Form | Liquid |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C Stable for one year after shipment. |
背景介绍
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |