验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
SINGLE CELL | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G65198-1-5C targets CD21 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
经测试应用 | Single Cell (Intra), Single Cell Application Description |
经测试反应性 | Human |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Oligo Conjugate |
产品类型 | Monoclonal |
全称 | MultiPro® 5CFLX Anti-Human CD21 (BU32) |
别名 | C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9 |
计算分子量 | 1092 aa, 119 kDa |
GenBank蛋白编号 | BC136394 |
基因名称 | CD21 |
Gene ID (NCBI) | 1380 |
ENSEMBL Gene ID | ENSG00000117322 |
RRID | AB_3673933 |
偶联类型 | 5CFLX |
完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCCACAATAATGTCAACCCATATAAGAAA |
条形码序列 | CCACAATAATGTCAA |
形式 | Liquid |
UNIPROT ID | P20023 |
储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |