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MultiPro™ 5CFLX Anti-Human CD29 (TS2/16)

CD29 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

TGAGAGACACGCATC

CloneNo.

TS2/16

Cat no : G65191-1-5C

Print datasheet

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

验证数据展示

  • Single Cell Sequencing experiment G65191-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65191-1-5C on Resting PBMC

    G65191-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65191-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65191-1-5C on PBMC treated with 4hr LPS + TI

    G65191-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65191-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65191-1-5C on Resting PBMC

    G65191-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65191-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65191-1-5C on PBMC treated with 4hr LPS + TI

    G65191-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65191-1-5C targets CD29 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD29 (TS2/16)
Synonyms CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB
Calculated Molecular Weight 88 kDa
GenBank Accession NumberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTGAGAGACACGCATCCCCATATAAGAAA
Barcode SequenceTGAGAGACACGCATC
Form Liquid
UNIPROT IDP05556
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol