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MultiPro™ 5CFLX Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCGGATAAGACCTTC

CloneNo.

OKT3

Cat no : G65133-1-5C

Print datasheet

Synonyms

CD3, CD3E, T3E, TCRE

验证数据展示

  • Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    G65133-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    G65133-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65133-1-5C targets CD3 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Human T cells 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD3 (OKT3)
Synonyms CD3, CD3E, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCGGATAAGACCTTCCCCATATAAGAAA
Barcode SequenceTCGGATAAGACCTTC
Form Liquid
UNIPROT IDP07766
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol