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验证数据展示

  • Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66065-2-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66065-2-5C on PBMC treated with 4hr LPS + TI

    G66065-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD31 (3F8E2)

CD31 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G66065-2-5C

产品说明书

CloneNo. 3F8E2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66065-2-5C targets CD31 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 CD31 fusion protein Ag19730 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD31 (3F8E2)
别名 CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker
计算分子量 83 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_3673942
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTATAACACCTGGTATCCCATATAAGAAA
条形码序列TATAACACCTGGTAT
形式 Liquid
UNIPROT IDP16284
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol