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MultiPro™ 5CFLX Anti-Human CD4 (RPA-T4)

CD4 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

GTCTTACAACGCAGT

CloneNo.

RPA-T4

Cat no : G65143-1-5C

Print datasheet

Synonyms

CD4, CD4 molecule, CD4mut

验证数据展示

  • Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    G65143-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    G65143-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65143-1-5C on Resting PBMC

    G65143-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65143-1-5C on PBMC treated with 4hr LPS + TI

    G65143-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65143-1-5C targets CD4 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD4 (RPA-T4)
Synonyms CD4, CD4 molecule, CD4mut
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGTCTTACAACGCAGTCCCATATAAGAAA
Barcode SequenceGTCTTACAACGCAGT
Form Liquid
UNIPROT IDP01730
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol