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验证数据展示

  • Single Cell Sequencing experiment G65103-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65103-1-5C on Resting PBMC

    G65103-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65103-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65103-1-5C on PBMC treated with 4hr LPS + TI

    G65103-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for Single Cell (Intra)
Cat No. G65103-1-5C

产品说明书

CloneNo. G28.5

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra)

别名

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65103-1-5C targets CD40 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD40 (G28.5)
别名 B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5
计算分子量 277 aa, 31 kDa
GenBank蛋白编号BC012419
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_3673913
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGATTGCTATGACGTACCCCATATAAGAAA
条形码序列ATTGCTATGACGTAC
形式 Liquid
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol