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MultiPro® 5CFLX Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

ATTGCTATGACGTAC

CloneNo.

G28.5

Cat no : G65103-1-5C

Print datasheet

Synonyms

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

验证数据展示

  • Single Cell Sequencing experiment G65103-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65103-1-5C on Resting PBMC

    G65103-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65103-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65103-1-5C on PBMC treated with 4hr LPS + TI

    G65103-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65103-1-5C targets CD40 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD40 (G28.5)
Synonyms B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5
Calculated Molecular Weight 277 aa, 31 kDa
GenBank Accession NumberBC012419
Gene Symbol CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGATTGCTATGACGTACCCCATATAAGAAA
Barcode SequenceATTGCTATGACGTAC
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol