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验证数据展示

  • Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    G66224-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    G66224-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD43 (2A11D6)

CD43 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66224-1-5C

Print datasheet

CloneNo. 2A11D6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

Single Cell (Intra)

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66224-1-5C targets CD43 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen CD43 fusion protein Ag23595 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD43 (2A11D6)
Synonyms CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN
Calculated Molecular Weight 400 aa, 43 kDa
GenBank Accession NumberBC012350
Gene Symbol CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_3673945
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCACAATCCTAACGGCCCATATAAGAAA
Barcode SequenceCCACAATCCTAACGG
Form Liquid
UNIPROT IDP16150
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol