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验证数据展示

  • Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    G66224-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    G66224-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD43 (2A11D6)

CD43 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66224-1-5C

产品说明书

CloneNo. 2A11D6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

Single Cell (Intra)

别名

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66224-1-5C targets CD43 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 CD43 fusion protein Ag23595 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD43 (2A11D6)
别名 CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN
计算分子量 400 aa, 43 kDa
GenBank蛋白编号BC012350
基因名称 CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_3673945
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGCCACAATCCTAACGGCCCATATAAGAAA
条形码序列CCACAATCCTAACGG
形式 Liquid
UNIPROT IDP16150
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol