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MultiProTM 5CFLX Anti-Human CD44 (F10-44-2)

CD44 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

AACAGCGTGCCGATT

CloneNo.

F10-44-2

Cat No : G65063-1-5C

Print datasheet

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

验证数据展示

  • Single Cell Sequencing experiment G65063-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65063-1-5C on Resting PBMC

    G65063-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65063-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65063-1-5C on PBMC treated with 4hr LPS + TI

    G65063-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65063-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65063-1-5C on Resting PBMC

    G65063-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65063-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65063-1-5C on PBMC treated with 4hr LPS + TI

    G65063-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65063-1-5C targets CD44 in applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell
Tested Reactivity Human
Immunogen Purified T cells from human lymph nodes 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiProTM 5CFLX Anti-Human CD44 (F10-44-2)
Synonyms CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
Calculated Molecular Weight 742 aa, 82 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAACAGCGTGCCGATTCCCATATAAGAAA
Barcode SequenceAACAGCGTGCCGATT
Form Liquid
UNIPROT IDP16070
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol