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MultiPro™ 5CFLX Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

TTACTCCTCCAGGTT

CloneNo.

F8-11-13

Cat no : G65226-1-5C

Print datasheet

Synonyms

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

验证数据展示

  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65226-1-5C targets CD45RA in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen Human T lymphocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD45RA (F8-11-13)
Synonyms B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Calculated Molecular Weight 1304 aa, 147 kDa
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTTACTCCTCCAGGTTCCCATATAAGAAA
Barcode SequenceTTACTCCTCCAGGTT
Form Liquid
UNIPROT IDP08575
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol