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验证数据展示

  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65226-1-5C on Resting PBMC

    G65226-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65226-1-5C on PBMC treated with 4hr LPS + TI

    G65226-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G65226-1-5C

产品说明书

CloneNo. F8-11-13

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65226-1-5C targets CD45RA in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 Human T lymphocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD45RA (F8-11-13)
别名 B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
计算分子量 1304 aa, 147 kDa
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3673937
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTTACTCCTCCAGGTTCCCATATAAGAAA
条形码序列TTACTCCTCCAGGTT
形式 Liquid
UNIPROT IDP08575
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol