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验证数据展示

  • Single Cell Sequencing experiment G65075-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65075-1-5C on Resting PBMC

    G65075-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65075-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65075-1-5C on PBMC treated with 4hr LPS + TI

    G65075-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD54/ICAM-1 (15.2)

CD54 (ICAM-1) Monoclonal Antibody for Single Cell (Intra)
Cat No. G65075-1-5C

产品说明书

CloneNo. 15.2

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra)

别名

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65075-1-5C targets CD54 (ICAM-1) in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD54/ICAM-1 (15.2)
别名 BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58
计算分子量 90 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_3673909
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGAGTGTTGTGGAGATCCCCATATAAGAAA
条形码序列AGTGTTGTGGAGATC
形式 Liquid
UNIPROT IDP05362
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol