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MultiPro® 5CFLX Anti-Human CD54/ICAM-1 (15.2)

CD54 (ICAM-1) Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

AGTGTTGTGGAGATC

CloneNo.

15.2

Cat no : G65075-1-5C

Print datasheet

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

验证数据展示

  • Single Cell Sequencing experiment G65075-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65075-1-5C on Resting PBMC

    G65075-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65075-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65075-1-5C on PBMC treated with 4hr LPS + TI

    G65075-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65075-1-5C targets CD54 (ICAM-1) in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD54/ICAM-1 (15.2)
Synonyms BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAGTGTTGTGGAGATCCCCATATAAGAAA
Barcode SequenceAGTGTTGTGGAGATC
Form Liquid
UNIPROT IDP05362
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol