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验证数据展示

  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G65253-1-5C

产品说明书

CloneNo. 10.1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65253-1-5C targets CD64 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD64 (10.1)
别名 CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I
计算分子量 374 aa, 43 kDa
GenBank蛋白编号BC032634
基因名称 FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
RRIDAB_3673939
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGCCATCGCCAGCGCGGCCCATATAAGAAA
条形码序列CCATCGCCAGCGCGG
形式 Liquid
UNIPROT IDP12314
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol