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MultiPro® 5CFLX Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

CCATCGCCAGCGCGG

CloneNo.

10.1

Cat no : G65253-1-5C

Print datasheet

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

验证数据展示

  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65253-1-5C on Resting PBMC

    G65253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65253-1-5C on PBMC treated with 4hr LPS + TI

    G65253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65253-1-5C targets CD64 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD64 (10.1)
Synonyms CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I
Calculated Molecular Weight 374 aa, 43 kDa
GenBank Accession NumberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCATCGCCAGCGCGGCCCATATAAGAAA
Barcode SequenceCCATCGCCAGCGCGG
Form Liquid
UNIPROT IDP12314
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol