MultiPro™ 5CFLX Anti-Human CD8 (SK1)
CD8 Monoclonal Antibody for Single Cell (Intra)
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
Single Cell (Intra)
Conjugate
5CFLX
Barcode Sequence
ATTAGATTGGTTAAC
CloneNo.
SK1
验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G65146-1-5C targets CD8 in Single Cell (Intra) applications and shows reactivity with Human samples.
Tested Applications | Single Cell (Intra) Application Description |
Tested Reactivity | Human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Oligo Conjugate |
Type | Monoclonal |
Full Name | MultiPro™ 5CFLX Anti-Human CD8 (SK1) |
Synonyms | CD8, CD8A, CD8a molecule, Leu2, MAL, p32, SK1 |
Calculated Molecular Weight | 235 aa, 26 kDa |
GenBank Accession Number | BC025715 |
Gene Symbol | CD8A |
Gene ID (NCBI) | 925 |
ENSEMBL Gene ID | ENSG00000153563 |
Conjugate | 5CFLX |
Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGATTAGATTGGTTAACCCCATATAAGAAA |
Barcode Sequence | ATTAGATTGGTTAAC |
Form | Liquid |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C Stable for one year after shipment. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |