验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G67722-1-5C targets GATA2 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | GATA2 fusion protein Ag1557 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human GATA2 (2B5A1) |
| 别名 | FLJ45948, GATA binding protein 2, GATA2, NFE1B |
| GenBank蛋白编号 | BC018988 |
| 基因名称 | GATA2 |
| Gene ID (NCBI) | 2624 |
| ENSEMBL Gene ID | ENSG00000179348 |
| RRID | AB_3673967 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTCACGCCGTCACGAGCCCATATAAGAAA |
| 条形码序列 | TCACGCCGTCACGAG |
| 形式 | Liquid |
| UNIPROT ID | P23769 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |