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验证数据展示

  • Single Cell Sequencing experiment G26477-1-5C on Resting PBMC

    Single Cell Sequencing experiment G26477-1-5C on Resting PBMC

    G26477-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G26477-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G26477-1-5C on PBMC treated with 4hr LPS + TI

    G26477-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human L-selectin/CD62L (Polyclonal)

L-selectin / CD62L Polyclonal Antibody for Single Cell (Intra)
Cat No. G26477-1-5C

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

Single Cell (Intra)

别名

CD62L, gp90 MEL, hLHRc, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, L-selectin, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G26477-1-5C targets L-selectin / CD62L in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 L-selectin / CD62L fusion protein Ag24063 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Oligo Conjugate
产品类型 Polyclonal
全称 MultiPro® 5CFLX Anti-Human L-selectin/CD62L (Polyclonal)
别名 CD62L, gp90 MEL, hLHRc, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, L-selectin, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1
计算分子量 42 kDa
GenBank蛋白编号BC020758
基因名称 CD62L
Gene ID (NCBI) 6402
ENSEMBL Gene IDENSG00000188404
RRIDAB_3673896
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGAGCTTCGCTGAACCGCCCATATAAGAAA
条形码序列AGCTTCGCTGAACCG
形式 Liquid
UNIPROT IDP14151
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol