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MultiPro® 5CFLX Anti-Human LC3 (5P12)

LC3 Uni-rAbTM Recombinant Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CTTGACAATCGTGAA

CloneNo.

5P12

Cat no : G81004-1-5C

Print datasheet

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3

验证数据展示

  • Single Cell Sequencing experiment G81004-1-5C on Resting PBMC

    Single Cell Sequencing experiment G81004-1-5C on Resting PBMC

    G81004-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G81004-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G81004-1-5C on PBMC treated with 4hr LPS + TI

    G81004-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G81004-1-5C targets LC3 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen LC3 fusion protein Ag6144 种属同源性预测
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Recombinant
Full Name MultiPro® 5CFLX Anti-Human LC3 (5P12)
Synonyms LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3
Calculated Molecular Weight 15 kDa
GenBank Accession NumberBC067797
Gene Symbol LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCTTGACAATCGTGAACCCATATAAGAAA
Barcode SequenceCTTGACAATCGTGAA
Form Liquid
UNIPROT IDQ9GZQ8
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol