MultiPro™ 5CFLX Anti-Human LC3 (5P12)
LC3 Uni-rAbTM Recombinant Antibody for Single Cell (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
Single Cell (Intra)
Conjugate
5CFLX
Barcode Sequence
CTTGACAATCGTGAA
CloneNo.
5P12
验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G81004-1-5C targets LC3 in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Applications | Single Cell (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | LC3 fusion protein Ag6144 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Oligo Conjugate |
| Type | Recombinant |
| Full Name | MultiPro™ 5CFLX Anti-Human LC3 (5P12) |
| Synonyms | LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3 |
| Calculated Molecular Weight | 15 kDa |
| GenBank Accession Number | BC067797 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCTTGACAATCGTGAACCCATATAAGAAA |
| Barcode Sequence | CTTGACAATCGTGAA |
| Form | Liquid |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
背景介绍
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |