验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G81004-1-5C targets LC3 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | LC3 fusion protein Ag6144 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Recombinant |
| 全称 | MultiPro® 5CFLX Anti-Human LC3 (5P12) |
| 别名 | LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3 |
| 计算分子量 | 15 kDa |
| GenBank蛋白编号 | BC067797 |
| 基因名称 | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_3673976 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCTTGACAATCGTGAACCCATATAAGAAA |
| 条形码序列 | CTTGACAATCGTGAA |
| 形式 | Liquid |
| UNIPROT ID | Q9GZQ8 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |