验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G66414-1-5C targets LDLR in Single Cell (Intra) applications and shows reactivity with Human samples.
| Tested Applications | Single Cell (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | LDLR fusion protein Ag1236 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Oligo Conjugate |
| Type | Monoclonal |
| Full Name | MultiPro® 5CFLX Anti-Human LDLR (1A2A3) |
| Synonyms | FH, FHC, LDL receptor, LDLR |
| Calculated Molecular Weight | 95 kDa |
| GenBank Accession Number | BC014514 |
| Gene Symbol | LDLR |
| Gene ID (NCBI) | 3949 |
| ENSEMBL Gene ID | ENSG00000130164 |
| RRID | AB_3673953 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGAGCAGGACTAACGAGCCCATATAAGAAA |
| Barcode Sequence | AGCAGGACTAACGAG |
| Form | Liquid |
| UNIPROT ID | P01130 |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
背景介绍
LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |