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MultiPro™ 5CFLX Anti-Human Lamin A/C (5I16)

Lamin A/C Uni-rAbTM Recombinant Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

AAGTGGAGTGTGTCT

CloneNo.

5I16

Cat no : G81042-1-5C

Print datasheet

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1

验证数据展示

  • Single Cell Sequencing experiment G81042-1-5C on Resting PBMC

    Single Cell Sequencing experiment G81042-1-5C on Resting PBMC

    G81042-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G81042-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G81042-1-5C on PBMC treated with 4hr LPS + TI

    G81042-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G81042-1-5C targets Lamin A/C in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Lamin A/C fusion protein Ag0408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Recombinant
Full Name MultiPro™ 5CFLX Anti-Human Lamin A/C (5I16)
Synonyms 70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1
Calculated Molecular Weight 65 kDa
GenBank Accession NumberBC003162
Gene Symbol Lamin A/C
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAAGTGGAGTGTGTCTCCCATATAAGAAA
Barcode SequenceAAGTGGAGTGTGTCT
Form Liquid
UNIPROT IDP02545
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol