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验证数据展示

  • Single Cell Sequencing experiment G20189-1-5C on Resting PBMC

    Single Cell Sequencing experiment G20189-1-5C on Resting PBMC

    G20189-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G20189-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G20189-1-5C on PBMC treated with 4hr LPS + TI

    G20189-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)

MAFB Polyclonal Antibody for Single Cell (Intra)
Cat No. G20189-1-5C

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

Single Cell (Intra)

别名

KRML, Maf B, MAFB, Transcription factor MafB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G20189-1-5C targets MAFB in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 MAFB fusion protein Ag14102 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Oligo Conjugate
产品类型 Polyclonal
全称 MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)
别名 KRML, Maf B, MAFB, Transcription factor MafB
计算分子量 323 aa, 36 kDa
GenBank蛋白编号BC036689
基因名称 MAFB
Gene ID (NCBI) 9935
ENSEMBL Gene IDENSG00000204103
RRIDAB_3673893
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTCGCGGACCAGGAATCCCATATAAGAAA
条形码序列TCGCGGACCAGGAAT
形式 Liquid
UNIPROT IDQ9Y5Q3
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide
储存条件2-8°C Stable for one year after shipment.

背景介绍

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol