验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G66920-1-5C targets NFKB2 in Single Cell (Intra) applications and shows reactivity with Human samples.
经测试应用 | Single Cell (Intra) Application Description |
经测试反应性 | Human |
免疫原 | NFKB2 fusion protein Ag28543 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Oligo Conjugate |
产品类型 | Monoclonal |
全称 | MultiPro® 5CFLX Anti-Human NFKB2 (6A10E9) |
别名 | DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100 |
计算分子量 | 97 kDa |
GenBank蛋白编号 | BC002844 |
基因名称 | NFKB2 |
Gene ID (NCBI) | 4791 |
ENSEMBL Gene ID | ENSG00000077150 |
RRID | AB_3673962 |
偶联类型 | 5CFLX |
完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCGAAGTGTGACATCTCCCATATAAGAAA |
条形码序列 | CGAAGTGTGACATCT |
形式 | Liquid |
UNIPROT ID | Q00653 |
储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |