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MultiPro™ 5CFLX Anti-Human NFKB2 (6A10E9)

NFKB2 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CGAAGTGTGACATCT

CloneNo.

6A10E9

Cat no : G66920-1-5C

Print datasheet

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100

验证数据展示

  • Single Cell Sequencing experiment G66920-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66920-1-5C on Resting PBMC

    G66920-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66920-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66920-1-5C on PBMC treated with 4hr LPS + TI

    G66920-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66920-1-5C targets NFKB2 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen NFKB2 fusion protein Ag28543 种属同源性预测
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human NFKB2 (6A10E9)
Synonyms DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100
Calculated Molecular Weight 97 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCGAAGTGTGACATCTCCCATATAAGAAA
Barcode SequenceCGAAGTGTGACATCT
Form Liquid
UNIPROT IDQ00653
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol