验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G66659-1-5C targets POU2AF1 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | POU2AF1 fusion protein Ag23613 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human POU2AF1 (3C5A7) |
| 别名 | BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1 |
| 计算分子量 | 256 aa, 27 kDa |
| GenBank蛋白编号 | BC032549 |
| 基因名称 | POU2AF1 |
| Gene ID (NCBI) | 5450 |
| ENSEMBL Gene ID | ENSG00000110777 |
| RRID | AB_3673958 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGGGTATCCGCAAGCGTCCCATATAAGAAA |
| 条形码序列 | GGTATCCGCAAGCGT |
| 形式 | Liquid |
| UNIPROT ID | Q16633 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |