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验证数据展示

  • Single Cell Sequencing experiment G66489-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66489-1-5C on Resting PBMC

    G66489-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66489-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66489-1-5C on PBMC treated with 4hr LPS + TI

    G66489-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human S100A4 (2G11B4)

S100A4 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66489-1-5C

产品说明书

CloneNo. 2G11B4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

Single Cell (Intra)

别名

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy S100A4 Ready-To-Use IHC Kit. S100A4 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66489-1-5C targets S100A4 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 S100A4 fusion protein Ag9019 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human S100A4 (2G11B4)
别名 18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4
计算分子量 101 aa, 12 kDa
GenBank蛋白编号BC016300
基因名称 S100A4
Gene ID (NCBI) 6275
ENSEMBL Gene IDENSG00000196154
RRIDAB_3673955
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGGTACCGCGCTCGAGACCCATATAAGAAA
条形码序列GTACCGCGCTCGAGA
形式 Liquid
UNIPROT IDP26447
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol