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MultiProTM 5CFLX Anti-Human SP1 (4G3H3)

SP1 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCATTGCGGATTAGC

CloneNo.

4G3H3

Cat No : G66508-1-5C

Print datasheet

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

验证数据展示

  • Single Cell Sequencing experiment G66508-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66508-1-5C on Resting PBMC

    G66508-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66508-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66508-1-5C on PBMC treated with 4hr LPS + TI

    G66508-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66508-1-5C targets SP1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen SP1 fusion protein Ag16720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Full Name MultiProTM 5CFLX Anti-Human SP1 (4G3H3)
Synonyms SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1
Calculated Molecular Weight 785 aa, 81 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCATTGCGGATTAGCCCCATATAAGAAA
Barcode SequenceTCATTGCGGATTAGC
Form Liquid
UNIPROT IDP08047
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol