验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G66508-1-5C targets SP1 in Single Cell (Intra) applications and shows reactivity with Human samples.
经测试应用 | Single Cell (Intra) Application Description |
经测试反应性 | Human |
免疫原 | SP1 fusion protein Ag16720 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Oligo Conjugate |
产品类型 | Monoclonal |
全称 | MultiPro® 5CFLX Anti-Human SP1 (4G3H3) |
别名 | SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1 |
计算分子量 | 785 aa, 81 kDa |
GenBank蛋白编号 | BC062539 |
基因名称 | SP1 |
Gene ID (NCBI) | 6667 |
ENSEMBL Gene ID | ENSG00000185591 |
RRID | AB_3673956 |
偶联类型 | 5CFLX |
完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTCATTGCGGATTAGCCCCATATAAGAAA |
条形码序列 | TCATTGCGGATTAGC |
形式 | Liquid |
UNIPROT ID | P08047 |
储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |