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验证数据展示

  • Single Cell Sequencing experiment G66618-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66618-2-5C on Resting PBMC

    G66618-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66618-2-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66618-2-5C on PBMC treated with 4hr LPS + TI

    G66618-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human SPI1 (2H3D3)

SPI1 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66618-2-5C

产品说明书

CloneNo. 2H3D3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

Single Cell (Intra)

别名

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66618-2-5C targets SPI1 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human SPI1 (2H3D3)
别名 31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1
计算分子量 31 kDa
GenBank蛋白编号NM_003120
基因名称 SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_3673957
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGGCAATAACCTCCGCGCCCATATAAGAAA
条形码序列GCAATAACCTCCGCG
形式 Liquid
UNIPROT IDP17947
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol