验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy TCF7 Ready-To-Use IHC Kit. TCF7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G14464-1-5C targets TCF7 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | TCF7 fusion protein Ag5792 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Polyclonal |
| 全称 | MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal) |
| 别名 | T cell factor 1, TCF 1, TCF 7, TCF1, TCF7, Transcription factor 7 |
| 计算分子量 | 42 kDa |
| GenBank蛋白编号 | BC048769 |
| 基因名称 | TCF1/TCF7 |
| Gene ID (NCBI) | 6932 |
| ENSEMBL Gene ID | ENSG00000081059 |
| RRID | AB_3673888 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCGCCTATAGAGACGCCCCATATAAGAAA |
| 条形码序列 | CGCCTATAGAGACGC |
| 形式 | Liquid |
| UNIPROT ID | P36402 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |