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验证数据展示

  • Single Cell Sequencing experiment G60291-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60291-1-5C on Resting PBMC

    G60291-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60291-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60291-1-5C on PBMC treated with 4hr LPS + TI

    G60291-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human TNF Alpha (7B8A11)

TNF Alpha Monoclonal Antibody for Single Cell (Intra)
Cat No. G60291-1-5C

产品说明书

CloneNo. 7B8A11

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

Single Cell (Intra)

别名

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G60291-1-5C targets TNF Alpha in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 TNF Alpha fusion protein Ag11413 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human TNF Alpha (7B8A11)
别名 Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor
计算分子量 233 aa, 26 kDa
GenBank蛋白编号BC028148
基因名称 TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_3673901
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGACACCGTATGATAGGCCCATATAAGAAA
条形码序列ACACCGTATGATAGG
形式 Liquid
UNIPROT IDP01375
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol