验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G67244-1-5C targets TPM4 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | TPM4 fusion protein Ag6947 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human TPM4 (2E12D12) |
| 别名 | TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain |
| 计算分子量 | 248 aa, 29 kDa |
| GenBank蛋白编号 | BC037576 |
| 基因名称 | TPM4 |
| Gene ID (NCBI) | 7171 |
| ENSEMBL Gene ID | ENSG00000167460 |
| RRID | AB_3673963 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTCACCGCATATATACCCCATATAAGAAA |
| 条形码序列 | TCACCGCATATATAC |
| 形式 | Liquid |
| UNIPROT ID | P67936 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |