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MultiPro™ 5CFLX Anti-Human Vimentin (Polyclonal)

Vimentin Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCCTAATCGTATTGC

Cat no : G10366-1-5C

Print datasheet

Synonyms

VIM, vimentin

验证数据展示

  • Single Cell Sequencing experiment G10366-1-5C on Resting PBMC

    Single Cell Sequencing experiment G10366-1-5C on Resting PBMC

    G10366-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G10366-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G10366-1-5C on PBMC treated with 4hr LPS + TI

    G10366-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G10366-1-5C targets Vimentin in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Full Name MultiPro™ 5CFLX Anti-Human Vimentin (Polyclonal)
Synonyms VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCCTAATCGTATTGCCCCATATAAGAAA
Barcode SequenceTCCTAATCGTATTGC
Form Liquid
UNIPROT IDP08670
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol