MultiPro® 5CFLX Anti-Human Vimentin (Polyclonal)
Vimentin Polyclonal Antibody for Single Cell (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
Single Cell (Intra)
Conjugate
5CFLX
Barcode Sequence
TCCTAATCGTATTGC
验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G10366-1-5C targets Vimentin in Single Cell (Intra) applications and shows reactivity with Human samples.
Tested Applications | Single Cell (Intra) Application Description |
Tested Reactivity | Human |
Immunogen | Vimentin fusion protein Ag0489 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Oligo Conjugate |
Type | Polyclonal |
Full Name | MultiPro® 5CFLX Anti-Human Vimentin (Polyclonal) |
Synonyms | VIM, vimentin |
Calculated Molecular Weight | 466 aa, 54 kDa |
GenBank Accession Number | BC000163 |
Gene Symbol | VIM |
Gene ID (NCBI) | 7431 |
ENSEMBL Gene ID | ENSG00000026025 |
Conjugate | 5CFLX |
Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGTCCTAATCGTATTGCCCCATATAAGAAA |
Barcode Sequence | TCCTAATCGTATTGC |
Form | Liquid |
UNIPROT ID | P08670 |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C Stable for one year after shipment. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |