验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G60200-1-5C targets ZAP70 in Single Cell (Intra) applications and shows reactivity with Human samples.
经测试应用 | Single Cell (Intra) Application Description |
经测试反应性 | Human |
免疫原 | ZAP70 fusion protein Ag7943 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Oligo Conjugate |
产品类型 | Monoclonal |
全称 | MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9) |
别名 | 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70 |
计算分子量 | 70 kDa |
GenBank蛋白编号 | BC039039 |
基因名称 | ZAP70 |
Gene ID (NCBI) | 7535 |
ENSEMBL Gene ID | ENSG00000115085 |
RRID | AB_3673899 |
偶联类型 | 5CFLX |
完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCTCTACCGCGCGACGCCCATATAAGAAA |
条形码序列 | CTCTACCGCGCGACG |
形式 | Liquid |
UNIPROT ID | P43403 |
储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide |
储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |