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MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)

ZAP70 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

CTCTACCGCGCGACG

CloneNo.

1A11G9

Cat no : G60200-1-5C

Print datasheet

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

验证数据展示

  • Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    G60200-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    G60200-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G60200-1-5C targets ZAP70 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)
Synonyms 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
Calculated Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCTCTACCGCGCGACGCCCATATAAGAAA
Barcode SequenceCTCTACCGCGCGACG
Form Liquid
UNIPROT IDP43403
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol