验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G60315-1-5C targets c-SRC in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | c-SRC fusion protein Ag22306 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human c-SRC (5E10C4) |
| 别名 | ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1 |
| 计算分子量 | 60 kDa |
| GenBank蛋白编号 | BC011566 |
| 基因名称 | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| RRID | AB_3673902 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGGGTACAAGGCTCATCCCCATATAAGAAA |
| 条形码序列 | GGTACAAGGCTCATC |
| 形式 | Liquid |
| UNIPROT ID | P12931 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |