验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, SGC-7901 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14844-1-AP targets Myh9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy polypeptide 9, non-muscle |
| 别名 | Myosin heavy chain, non-muscle IIa, Myhn1, Myhn 1, D0Jmb2, Cellular myosin heavy chain, type A |
| 计算分子量 | 226 kDa |
| 观测分子量 | 224 kDa |
| GenBank蛋白编号 | NM_022410 |
| 基因名称 | Myh9 |
| Gene ID (NCBI) | 17886 |
| RRID | AB_2147311 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8VDD5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH9, also named as NMMHC-A and NMMHC-IIA, is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. It plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, during cell spreading.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Myh9 antibody 14844-1-AP | Download protocol |
| IHC protocol for Myh9 antibody 14844-1-AP | Download protocol |
| IF protocol for Myh9 antibody 14844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells. | ||
Theranostics Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.
| ||
Neuromolecular Med Generation and characterization of mice with Myh9 deficiency.
| ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Biomed Pharmacother Tandem mass tag (TMT) proteomic analysis of fetal lungs revealed differential expression of tight junction proteins in a rat model of congenital diaphragmatic hernia. |
