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  • Featured Product
  • KD/KO Validated

GLYR1 Polyclonal antibody

GLYR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 14833-1-AP

Print datasheet

Synonyms

NDF, hNDF, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC

验证数据展示

  • WB analysis using 14833-1-AP

    WB analysis using 14833-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14833-1-AP (GLYR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14833-1-AP

    IHC staining of mouse testis using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLYR1 antibody included
  • IHC staining of mouse testis using 14833-1-AP

    IHC staining of mouse testis using 14833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14833-1-AP (GLYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLYR1 antibody included
  • IF Staining of HeLa using 14833-1-AP

    IF Staining of HeLa using 14833-1-AP

    Immunofluorescent analysis of Hela cells, using N-PAC antibody 14833-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of K-562 using 14833-1-AP

    IP experiment of K-562 using 14833-1-AP

    IP result of anti-GLYR1 (IP:14833-1-AP, 4ug; Detection:14833-1-AP 1:500) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy GLYR1 Ready-To-Use IHC Kit. GLYR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14833-1-AP targets GLYR1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, ChIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GLYR1 fusion protein Ag6637 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytokine-like nuclear factor n-pac
Synonyms NDF, hNDF, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC064940
Gene Symbol N-PAC
Gene ID (NCBI) 84656
RRIDAB_10859775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ49A26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLYR1 antibody 14833-1-APDownload protocol
IHC protocol for GLYR1 antibody 14833-1-APDownload protocol
IF protocol for GLYR1 antibody 14833-1-APDownload protocol
IP protocol for GLYR1 antibody 14833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell

Transcription factor protein interactomes reveal genetic determinants in heart disease.

Authors - Barbara Gonzalez-Teran
View Article
mouseChIP, WB

Genomics Proteomics Bioinformatics

Npac Is a Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells.

Authors - Sue Yu
View Article
humanWB,IHC,CoIP,IF

J Exp Clin Cancer Res

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Authors - Zhiyan Hu
  • KD Validated
View Article