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N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30803-1-AP

Print datasheet

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

验证数据展示

  • WB analysis using 30803-1-AP

    WB analysis using 30803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30803-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 30803-1-AP

    IHC staining of mouse heart using 30803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of mouse heart using 30803-1-AP

    IHC staining of mouse heart using 30803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of C2C12 using 30803-1-AP

    IF Staining of C2C12 using 30803-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using N-cadherin antibody (30803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, PC-3 cells, C2C12 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30803-1-AP targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19022-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

实验方案

Product Specific Protocols
WB protocol for N-cadherin antibody 30803-1-APDownload protocol
IHC protocol for N-cadherin antibody 30803-1-APDownload protocol
IF protocol for N-cadherin antibody 30803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols