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N-cadherin Monoclonal antibody, PBS Only

N-cadherin Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2G7C2

Cat No : 68532-1-PBS

Print datasheet

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

验证数据展示

  • WB analysis using 68532-1-Ig (same clone as 68532-1-PBS)

    WB analysis using 68532-1-Ig (same clone as 68532-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

68532-1-PBS targets N-cadherin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19022-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).