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Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody

N-cadherin/CD325 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230259D7

Cat no : 98006-1-RR

Print datasheet

Synonyms

N-cadherin, CDHN, CDH2, CD325, Cadherin-2

验证数据展示

  • FC experiment of HeLa using 98006-1-RR

    FC experiment of HeLa using 98006-1-RR

    1x10^6 HeLa cells (left) or MCF-7 cells (right) were surface stained with 0.25 ug Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody (98006-1-RR, Clone: 230259D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98006-1-RR

    Affinity and Kinetic Characterization of 98006-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98006-1-RR against Human N-cadherin were performed. The affinity constant is 2.73 nM.

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98006-1-RR targets N-cadherin/CD325 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms N-cadherin, CDHN, CDH2, CD325, Cadherin-2
Calculated Molecular Weight100 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19022-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

实验方案

Product Specific Protocols
FC protocol for N-cadherin/CD325 antibody 98006-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols