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验证数据展示

  • WB analysis using 16733-1-AP

    WB analysis using 16733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16733-1-AP (N4BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16733-1-AP

    IHC staining of mouse kidney using 16733-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16733-1-AP

    IHC staining of mouse kidney using 16733-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 16733-1-AP

    IF Staining of HEK-293 using 16733-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IP experiment of mouse brain using 16733-1-AP

    IP experiment of mouse brain using 16733-1-AP

    IP result of anti-N4BP3 (IP:16733-1-AP, 4ug; Detection:16733-1-AP 1:2000) with mouse brain tissue lysate 1800 ug.

  • IF Staining of HEK-293 using 16733-1-AP

    IF Staining of HEK-293 using 16733-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

N4BP3 Polyclonal antibody

N4BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16733-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue, human cervical cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16733-1-AP targets N4BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen N4BP3 fusion protein Ag10194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Nedd4 binding protein 3
Synonyms NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC053323
Gene Symbol N4BP3
Gene ID (NCBI) 23138
RRIDAB_2878308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15049
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

实验方案

Product Specific Protocols
WB protocol for N4BP3 antibody 16733-1-APDownload protocol
IHC protocol for N4BP3 antibody 16733-1-APDownload protocol
IF protocol for N4BP3 antibody 16733-1-APDownload protocol
IP protocol for N4BP3 antibody 16733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Front Microbiol

N4BP3 Regulates RIG-I-Like Receptor Antiviral Signaling Positively by Targeting Mitochondrial Antiviral Signaling Protein.

Authors - Chen Wang
  • KD Validated
View Article
humanWB,IP,IHC

Cell Death Discov

N4BP3 facilitates NOD2-MAPK/NF-κB pathway in inflammatory bowel disease through mediating K63-linked RIPK2 ubiquitination

Authors - Wang Jiang
View Article