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  • Featured Product
  • KD/KO Validated

N4BP3 Polyclonal antibody

N4BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16733-1-AP

Print datasheet

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341

验证数据展示

  • WB analysis using 16733-1-AP

    WB analysis using 16733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16733-1-AP (N4BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16733-1-AP

    IHC staining of mouse kidney using 16733-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16733-1-AP

    IHC staining of mouse kidney using 16733-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16733-1-AP (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 16733-1-AP

    IF Staining of HEK-293 using 16733-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IP experiment of mouse brain using 16733-1-AP

    IP experiment of mouse brain using 16733-1-AP

    IP result of anti-N4BP3 (IP:16733-1-AP, 4ug; Detection:16733-1-AP 1:2000) with mouse brain tissue lysate 1800 ug.

  • IF Staining of HEK-293 using 16733-1-AP

    IF Staining of HEK-293 using 16733-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using N4BP3 antibody (16733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue, human cervical cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16733-1-AP targets N4BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen N4BP3 fusion protein Ag10194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Nedd4 binding protein 3
Synonyms NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC053323
Gene Symbol N4BP3
Gene ID (NCBI) 23138
RRIDAB_2878308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15049
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

实验方案

Product Specific Protocols
WB protocol for N4BP3 antibody 16733-1-APDownload protocol
IHC protocol for N4BP3 antibody 16733-1-APDownload protocol
IF protocol for N4BP3 antibody 16733-1-APDownload protocol
IP protocol for N4BP3 antibody 16733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Front Microbiol

N4BP3 Regulates RIG-I-Like Receptor Antiviral Signaling Positively by Targeting Mitochondrial Antiviral Signaling Protein.

Authors - Chen Wang
  • KD Validated
View Article