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NAAA Polyclonal antibody

NAAA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16576-1-AP

Print datasheet

Synonyms

ASAH-like protein, ASAHL, ASAH like protein, Acylsphingosine deacylase NAAA, Acid ceramidase-like protein

验证数据展示

  • WB analysis using 16576-1-AP

    WB analysis using 16576-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16576-1-AP (NAAA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16576-1-AP targets NAAA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen NAAA fusion protein Ag9861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acylethanolamine acid amidase
Synonyms ASAH-like protein, ASAHL, ASAH like protein, Acylsphingosine deacylase NAAA, Acid ceramidase-like protein
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC011854
Gene Symbol NAAA
Gene ID (NCBI) 27163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAAA (N-acylethanolamine acid amidase), also named ASAHL and PLT, is a novel lysosomal hydrolase structurally and functionally similar to acid ceramidase and is mainly involved in the hydrolysis of fatty amides. NAAA is mainly responsible for the degradation of bioactive fatty amides, such as palmitoylethanolamide (PEA), and is involved in lipid metabolism and fatty acid metabolism processes (PMID: 32191459; PMID: 17712833).

实验方案

Product Specific Protocols
WB protocol for NAAA antibody 16576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols