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验证数据展示

  • WB analysis of human heart using 19601-1-AP

    WB analysis of human heart using 19601-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19601-1-AP

    IHC staining of human brain using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 19601-1-AP

    IHC staining of human brain using 19601-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 19601-1-AP

    IP experiment of K-562 using 19601-1-AP

    IP result of anti-NAB2 (IP:19601-1-AP, 4ug; Detection:19601-1-AP 1:700) with K-562 cells lysate 3200ug.

  • IF Staining of MCF-7 using 19601-1-AP

    IF Staining of MCF-7 using 19601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NAB2 antibody (19601-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red).

NAB2 Polyclonal antibody

NAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19601-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Protein MADER, NGFI-A-binding protein 2, Nab 2, Melanoma-associated delayed early response protein, MADER

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, human brain tissue, K-562 cells, mouse thymus tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19601-1-AP targets NAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 NAB2 fusion protein Ag6346 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NGFI-A binding protein 2 (EGR1 binding protein 2)
别名 Protein MADER, NGFI-A-binding protein 2, Nab 2, Melanoma-associated delayed early response protein, MADER
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC065931
基因名称 NAB2
Gene ID (NCBI) 4665
RRIDAB_10640524
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAB2 antibody 19601-1-APDownload protocol
IHC protocol for NAB2 antibody 19601-1-APDownload protocol
IF protocol for NAB2 antibody 19601-1-APDownload protocol
IP protocol for NAB2 antibody 19601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Invest Ophthalmol Vis Sci

β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis.

Authors - Yichen Wang
View Article
mouseWB

Lab Anim (NY)

Early growth response 2 in the mPFC regulates mouse social and cooperative behaviors

Authors - Yanli Zhang
View Article