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NAB2 Monoclonal antibody

NAB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F1E2

Cat no : 68322-1-Ig

Print datasheet

Synonyms

EGR 1 binding protein 2, MADER, NAB2, NGFI A binding protein 2, Protein MADER

验证数据展示

  • WB analysis using 68322-1-Ig

    WB analysis using 68322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68322-1-Ig (NAB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68322-1-Ig

    WB analysis using 68322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68322-1-Ig (NAB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, A2780 cells, A375 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68322-1-Ig targets NAB2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen NAB2 fusion protein Ag6346 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name NGFI-A binding protein 2 (EGR1 binding protein 2)
Synonyms EGR 1 binding protein 2, MADER, NAB2, NGFI A binding protein 2, Protein MADER
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC065931
Gene Symbol NAB2
Gene ID (NCBI) 4665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15742
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NGFI-A-Binding protein 2 (NAB2) is a member of the poly(A)-binding protein (PABP) family in S. cerevisiae and emerged as a key component in the tightly intertwined system of poly(A) tail length control, mRNP formation, and export of mature mRNPs.

实验方案

Product Specific Protocols
WB protocol for NAB2 antibody 68322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols