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NADK Polyclonal antibody

NADK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15548-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, RAW264.7
Positive IHC detected inhuman kidney tissue, human testis tissue, human brain tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15548-1-AP targets NADK in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NADK fusion protein Ag7906 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NAD kinase
别名 Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC001709
基因名称 NADK
Gene ID (NCBI) 65220
RRIDAB_2147345
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

实验方案

Product Specific Protocols
WB protocol for NADK antibody 15548-1-APDownload protocol
IHC protocol for NADK antibody 15548-1-APDownload protocol
IF protocol for NADK antibody 15548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF

Cell Metab

Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.

Authors - Yang Zhang
  • KD Validated
humanIHC

Sci Adv

G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.

Authors - Yang Zhang
WB

Nucleic Acids Res

Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.

Authors - Shih-Hsun Chen
humanIHC

Technol Cancer Res Treat

A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer

Authors - Linjing Li
humanIF

Nat Commun

NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression.

Authors - Chunjing Bian
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
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