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验证数据展示

  • WB analysis of K-562 using 27764-1-AP

    WB analysis of K-562 using 27764-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27764-1-AP (NAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27764-1-AP

    IHC staining of human ovary cancer using 27764-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAF1 Polyclonal antibody

NAF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27764-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

hNAF1, H/ACA ribonucleoprotein complex non-core subunit NAF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27764-1-AP targets NAF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NAF1 fusion protein Ag26787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear assembly factor 1 homolog (S. cerevisiae)
别名 hNAF1, H/ACA ribonucleoprotein complex non-core subunit NAF1
计算分子量 54 kDa
观测分子量 70 kDa
GenBank蛋白编号BC008207
基因名称 NAF1
Gene ID (NCBI) 92345
RRIDAB_2880965
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HR8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAF1, an H/ACA box RNP assembly chaperone, is a factor necessary for H/ACA snoRNP maturation and ribosome biosynthesis in mammalian cells. In addition, among these RNA biogenesis factors, NAF1 may be unique in that it is required at full dosage for telomerase and telomere maintenance in the process of pulmonary fibrosis-emphysema. (PMID: 30936423)

实验方案

Product Specific Protocols
WB protocol for NAF1 antibody 27764-1-APDownload protocol
IHC protocol for NAF1 antibody 27764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols