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NAMPT/PBEF Polyclonal antibody
NAMPT/PBEF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, Ramos cells, mouse heart tissue, HL-60 cells, RAW 264.7 cells, mouse liver tissue, mouse skeletal muscle tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells, HeLa cells, U-87 MG cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11776-1-AP targets NAMPT/PBEF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, zebrafish |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | NAMPT/PBEF fusion protein Ag2434 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nicotinamide phosphoribosyltransferase |
Synonyms | Nampt, Visfatin, Pre B cell enhancing factor, PBEF1, PBEF |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC020691 |
Gene Symbol | NAMPT/PBEF |
Gene ID (NCBI) | 10135 |
RRID | AB_2298317 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P43490 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
IHC protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
IF protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
IP protocol for NAMPT/PBEF antibody 11776-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage. | ||
Cell Metab NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.
| ||
Cell Death Dis Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy | ||
Int J Mol Sci NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy. | ||
Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.
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Oncogenesis Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.
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