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验证数据展示

  • WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NAMPT antibody included
  • IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NAMPT antibody included
  • IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

    IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NAMPT/PBEF Monoclonal antibody, PBS Only

NAMPT/PBEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66385-1-PBS

产品说明书

CloneNo. 3D4D8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NAMPT Ready-To-Use IHC Kit. NAMPT primary antibody included.

产品信息

66385-1-PBS targets NAMPT/PBEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NAMPT/PBEF fusion protein Ag2434 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nicotinamide phosphoribosyltransferase
别名 1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020691
基因名称 NAMPT/PBEF
Gene ID (NCBI) 10135
RRIDAB_2881761
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43490
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.