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  • Featured Product
  • KD/KO Validated

NAMPT/PBEF Monoclonal antibody, PBS Only

NAMPT/PBEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D4D8

Cat no : 66385-1-PBS

Print datasheet

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

验证数据展示

  • WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NAMPT antibody included
  • IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NAMPT antibody included
  • IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

    IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NAMPT Ready-To-Use IHC Kit. NAMPT primary antibody included.

产品信息

66385-1-PBS targets NAMPT/PBEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NAMPT/PBEF fusion protein Ag2434 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nicotinamide phosphoribosyltransferase
Synonyms 1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC020691
Gene Symbol NAMPT/PBEF
Gene ID (NCBI) 10135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.