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Conjugate
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Conjugate
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NANOG Monoclonal antibody, PBS Only

NANOG Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A2E1

Cat no : 67255-1-PBS

Print datasheet

Synonyms

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

验证数据展示

  • WB analysis using 67255-1-Ig (same clone as 67255-1-PBS)

    WB analysis using 67255-1-Ig (same clone as 67255-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67255-1-Ig (NANOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67255-1-PBS in a different storage buffer formulation.

产品信息

67255-1-PBS targets NANOG in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NANOG fusion protein Ag21364 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Nanog homeobox
Synonyms hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC160187
Gene Symbol NANOG
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9S0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)