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- KD/KO Validated
NAP1L1 Polyclonal antibody
NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HT-1080 cells, A431 cells, HEK-293 cells, mouse thymus tissue, HL-60 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy NAP1L1 Ready-To-Use IHC Kit. NAP1L1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | NAP1L1 fusion protein Ag6690 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nucleosome assembly protein 1-like 1 |
| Synonyms | Nucleosome assembly protein 1-like 1, NRP, NAP-1-related protein, NAP1L, NAP1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC002387 |
| Gene Symbol | NAP1L1 |
| Gene ID (NCBI) | 4673 |
| RRID | AB_2150696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55209 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IHC protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IF protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IP protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.
| ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
J Virol NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation. | ||
Cancer Cell Int NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.
| ||
J Dermatol Sci Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells. | ||
