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NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27889-1-AP

Print datasheet

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

验证数据展示

  • WB analysis using 27889-1-AP

    WB analysis using 27889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27889-1-AP

    WB analysis of HeLa using 27889-1-AP

    WB result of NAP1L4 antibody (27889-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, HuH-7 cells, mouse heart tissue, mouse testis tissue, rat heart tissue, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27889-1-AP targets NAP1L4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NAP1L4 fusion protein Ag27469 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleosome assembly protein 1-like 4
Synonyms hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC022090
Gene Symbol NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2881006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

实验方案

Product Specific Protocols
WB protocol for NAP1L4 antibody 27889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols