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验证数据展示

  • WB analysis using 10546-1-AP

    WB analysis using 10546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (alpha-SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 10546-1-AP

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10546-1-AP

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha SNAP Polyclonal antibody

Alpha SNAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10546-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

alpha-SNAP, NAPA, SNAP alpha, SNAPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10546-1-AP targets Alpha SNAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha SNAP fusion protein Ag0836 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor attachment protein, alpha
别名 alpha-SNAP, NAPA, SNAP alpha, SNAPA
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC007432
基因名称 Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2150721
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAPs (soluble NSF-attachment proteins) may play an important role in the process of docking and fusion of vesicles to their target membranes, according to the 'SNARE hypothesis'. NAPA(Alpha- SNAP) was found in a wide range of tissues and act synergistically in intra-Golgi transport. This antibody recognizes a 33kd protein in SDS page.

实验方案

Product Specific Protocols
WB protocol for Alpha SNAP antibody 10546-1-APDownload protocol
IHC protocol for Alpha SNAP antibody 10546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols