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验证数据展示

  • WB analysis using 10546-1-AP

    WB analysis using 10546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (alpha-SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 10546-1-AP

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10546-1-AP

    IHC staining of mouse brain using 10546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha SNAP Polyclonal antibody

Alpha SNAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10546-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

alpha-SNAP, NAPA, SNAP alpha, SNAPA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10546-1-AP targets Alpha SNAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Alpha SNAP fusion protein Ag0836 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Synonyms alpha-SNAP, NAPA, SNAP alpha, SNAPA
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC007432
Gene Symbol Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2150721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAPs (soluble NSF-attachment proteins) may play an important role in the process of docking and fusion of vesicles to their target membranes, according to the 'SNARE hypothesis'. NAPA(Alpha- SNAP) was found in a wide range of tissues and act synergistically in intra-Golgi transport. This antibody recognizes a 33kd protein in SDS page.

实验方案

Product Specific Protocols
WB protocol for Alpha SNAP antibody 10546-1-APDownload protocol
IHC protocol for Alpha SNAP antibody 10546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols