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验证数据展示

  • WB analysis using 15604-1-AP

    WB analysis using 15604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15604-1-AP

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAPG antibody included
  • IHC staining of human ovary tumor using 15604-1-AP

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAPG antibody included
  • IP experiment of HepG2 using 15604-1-AP

    IP experiment of HepG2 using 15604-1-AP

    IP result of anti-NAPG (IP:15604-1-AP, 4ug; Detection:15604-1-AP 1:400) with HepG2 cells lysate 3300 ug.

NAPG Polyclonal antibody

NAPG Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15604-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

SNAP-gamma, SNAPG, SNAP gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, Gamma-soluble NSF attachment protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, K-562 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NAPG Ready-To-Use IHC Kit. NAPG primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15604-1-AP targets NAPG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 NAPG fusion protein Ag7988 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor attachment protein, gamma
别名 SNAP-gamma, SNAPG, SNAP gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, Gamma-soluble NSF attachment protein
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC001889
基因名称 NAPG
Gene ID (NCBI) 8774
RRIDAB_2150885
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99747
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAPG antibody 15604-1-APDownload protocol
IHC protocol for NAPG antibody 15604-1-APDownload protocol
IP protocol for NAPG antibody 15604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols