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NAPG Polyclonal antibody

NAPG Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15604-1-AP

Print datasheet

Synonyms

SNAP-gamma, SNAPG, SNAP gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, Gamma-soluble NSF attachment protein

验证数据展示

  • WB analysis using 15604-1-AP

    WB analysis using 15604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15604-1-AP

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAPG antibody included
  • IHC staining of human ovary tumor using 15604-1-AP

    IHC staining of human ovary tumor using 15604-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAPG antibody included
  • IP experiment of HepG2 using 15604-1-AP

    IP experiment of HepG2 using 15604-1-AP

    IP result of anti-NAPG (IP:15604-1-AP, 4ug; Detection:15604-1-AP 1:400) with HepG2 cells lysate 3300 ug.

经过测试的应用

Positive WB detected inA375 cells, K-562 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NAPG Ready-To-Use IHC Kit. NAPG primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15604-1-AP targets NAPG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen NAPG fusion protein Ag7988 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-ethylmaleimide-sensitive factor attachment protein, gamma
Synonyms SNAP-gamma, SNAPG, SNAP gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, Gamma-soluble NSF attachment protein
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001889
Gene Symbol NAPG
Gene ID (NCBI) 8774
RRIDAB_2150885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAPG antibody 15604-1-APDownload protocol
IHC protocol for NAPG antibody 15604-1-APDownload protocol
IP protocol for NAPG antibody 15604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols